Analysis

Spike2 has built-in detection of waveform features, measurements of latencies, amplitudes and areas as well as averages and trend plots. Further custom analysis functions can be applied via a script language.

• Generate tachogram channels based on detected waveform complex segments, such as ECG R-waves for heart rate variability studies
• Locate repolarisation percentages and measure latencies from cardiac action potentials
• Measure systolic and diastolic blood pressures on a beat-by-beat basis. Use detected peaks and troughs to calculate and plot mean blood pressure
• Mark and measure responses to drug applications

Waveform processing

Spike2 can apply channel processes (for example, rectification and smoothing) on-line and off-line to aid with the detection and measurement of waveform features. Virtual channels allow inter-channel arithmetic, such as calculating the third lead in Einthoven's triangle.

• Locate and mark points of interest in processed data. Use these reference markers to take measurements from the original signal
• Data processing options include digital filtering and smoothing to reduce noise and unwanted frequencies, differentiation to enable detection of hard to find components, and DC remove to normalise the signal and minimise baseline drift
• Applied processes only affect the displayed data, not the original stored on disk
• Create duplicate channels to show raw and processed data simultaneously

Measurements

Results from cursor positions, correlations, averages and script driven functions can be plotted and exported for further analysis, if required.

• Available measurements include: mean waveform value, slope, curve area, ratios, differences, and peak-to-peak amplitude
• Take multiple measurements based on cursor positions
• Plot results to new channels in the data file or XY scatter plots
• Export results to spreadsheet