Spike2 Scripts


Control a Telemetry Research interface (05/10)

Control a Telemetry Research interface

TR Control.zip

This script and associated sequence is an example of interacting with a Telemetry Research interface. The script creates a toolbar which allows the user to control sampling and set the duration of recording periods and gaps.

Digital output 0 of the 1401 should be connected to the trigger input of the Telemetry Research transmitter and DAC 0 connected to the external channel select input to select a channel between 1 and 12. The signal you want to sample should then be connected to ADC 0.

This script requires Spike2 v5.17 or higher.

Control a Digitimer DS5 Stimulator Improved! (01/17)

Control a Digitimer DS5 Stimulator


This script and associated sequence is an example of using a DAC output from the 1401 to control a Digitimer DS5 Isolated Bipolar Constant Current stimulator and update the settings during sampling.

This script requires Spike2 v6.14 or higher.

Calibrate waveform capture Improved! (01/17)

Calibrate waveform capture


This script allows the user to calibrate the scale and offset of waveform channels in a sampling configuration. First create a sampling configuration (or load in a previous one) and then run the script. You can sample data into a temporary data file. When sampling this data, you should make the waveform that you wish to calibrate stay at two distinct known levels for a short period of time.

You can then press the "Calibrate" button. This enables you to select the two level sections of waveform data in a channel and to specify what values they SHOULD be. The script will then alter the channel scale and offset in the sampling configuration to achieve those values.

Generate paired pulses and analyse the results
Improved! (01/17)

Generate paired pulses and analyse the results


The PPulse script sends paired pulses out through Digital Outputs 0 and 1. The time between the pulses (the inter-stimulus time) is chosen at random from a list of possible values held in a stipulated text file.

To run this script, make sure you have the sequencer files PPulse1, PPulse2 and PPulse3 in the same directory as the PPulse script. You must also have in this directory a text file containing the ISI's to use (an example text file called ISITimes is supplied).

Finally, connect Digital Outputs 0 and 1 to Event Inputs 0 and 1 on a micro 1401 (or if you are using a standard 1401 or 1401plus, connect Digital Outputs 0 and 1 to Digital Inputs 0 and 1).

The PPulseAn script is a standard analysis script which indicates how the analysis of data collected using the PPulse script might be automated.

Extended documentation (in Word format) is given for both scripts.

Some of these scripts have come from users rather than the CED team. If you have a script that you would like to offer to fellow users via this page, please tell Simon Gray. We provide some scripts for Signal too.

These scripts are stored as WinZip files, myscript.zip, except where they are shown as spike\scripts\myscript.s2s. Those latter files were installed with Spike2 and spike stands for the directory in which you installed Spike2. See the summaries by clicking on the description in the side menu. Then you can down-load them by clicking on the filename.


Mains ‘hum’ is often complex and composed of odd harmonics of the mains frequency, making it very difficult to remove or suppress using simple high pass or notch filters. HumRemoveExpress.s2s is a Spike2 version 7 script that you can use offline to strip out much of this residual mains interference, making your data much more presentable and easier to analyse. This video tutorial shows how to use the script to remove mains hum.

  • Alpha MED Sciences
  • Alpha Omega Engineering
  • Axon Instruments
  • Binary data
  • Bionic/Cyberkinetics
  • Biopac
  • CED SON (Macintosh)
  • CONSAM (D. Colquhuon)
  • DATAQ Instruments (Codas)
  • DataWave
  • Data Sciences International
  • Delsys Files
  • European Data Format(+)
  • Grass-Telefactor (PolyView)
  • HLR Data Format
  • Heka Data Format
  • Multi Channel System (Mc_Rack)
  • MindSet (16/24) data Files
  • MindWare data files
  • Motion Labs Systems Files .c3d
  • NeuroScan
  • Neuralynx
  • Plexon
  • RC Electronics
  • Text files
  • TMS International
  • Tucker-Davis Technologies
  • WAV (Microsoft)
  • WaveMetrics Igor Pro (PC/Mac)
  • Xltek Neuroworks
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